SidaGhanaV1, Main, Exploration, bibRecord, 000E26

Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form 02_AG (CRF02_AG) has a higher in vitro replicative capacity than its parental subtypes A and G

Identifieur interne : 000E26 ( Main/Exploration ); précédent : 000E25; suivant : 000E27

Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form 02_AG (CRF02_AG) has a higher in vitro replicative capacity than its parental subtypes A and G

Auteurs : Frank A. J. Konings [États-Unis] ; Sherri T. Burda [États-Unis] ; Mateusz M. Urbanski [États-Unis] ; Ping Zhong [République populaire de Chine] ; Arthur Nadas [États-Unis] ; Phillipe N. Nyambi [États-Unis]

Source :

Journal of Medical Virology [ 0146-6615 ] ; 2006-05.

RBID : ISTEX:A113A23D17C4AC3A743C615B17AE8F2A0E7E62B5

Descripteurs français

Wicri :
- geographic : Cameroun.

English descriptors

KwdEn :
- Aids research, Aids rrrp, Assay, Biological properties, Bootstrap, Bootstrap value, Burda, Cameroon, Cameroonian, Carr, Coreceptor usage, Culture medium, Cytotoxicity, Disease progression, Genome, Grant number, Grant sponsor, Growth medium, Heyndrickx, Higher replicative capacity, Human virus type, Infectivity, Konings, Molecular epidemiology, Nyambi, Other subtypes, Parental, Parental subtypes, Pbmcs, Phylogenetic, Phylogenetic analysis, Recombinant, Recombinant form, Recombination, Recombination breakpoint analysis, Recombination event, Reference reagent program, Replication, Replication rate, Replication rates, Replicative, Replicative capacity, Retrovirus, Rrrp, Sequence database, Subtype, Subtypes, Time point, Tness, Tted, Tted lines, Viral, Viral stock, Virol, Virus, Virus particles, Virus type, York university school, Zhong.
Teeft :
- Aids research, Aids rrrp, Assay, Biological properties, Bootstrap, Bootstrap value, Burda, Cameroon, Cameroonian, Carr, Coreceptor usage, Culture medium, Cytotoxicity, Disease progression, Genome, Grant number, Grant sponsor, Growth medium, Heyndrickx, Higher replicative capacity, Human virus type, Infectivity, Konings, Molecular epidemiology, Nyambi, Other subtypes, Parental, Parental subtypes, Pbmcs, Phylogenetic, Phylogenetic analysis, Recombinant, Recombinant form, Recombination, Recombination breakpoint analysis, Recombination event, Reference reagent program, Replication, Replication rate, Replication rates, Replicative, Replicative capacity, Retrovirus, Rrrp, Sequence database, Subtype, Subtypes, Time point, Tness, Tted, Tted lines, Viral, Viral stock, Virol, Virus, Virus particles, Virus type, York university school, Zhong.

Abstract

Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form (CRF) 02_AG is the predominant subtype in Cameroon, even more prevalent than the parental subtypes A and G. An important question that needs to be addressed is whether recombination in HIV‐1 infection can lead to the emergence of viruses with biological advantages. The replicative capacity was investigated in peripheral blood mononuclear cells (PBMCs) of 13 R5‐tropic primary HIV‐1 isolates, including 5 CRF02_AG, 4 subtype A, and 4 subtype G viruses. HIV‐1 subtype identity was defined by phylogeny either of the full‐length genome or analysis of a combination of segments of the gag, pro, pol, and env genes followed by recombination breakpoint analysis. All viruses were grown on PBMCs for 11 days and culture supernatant was analyzed for reverse transcriptase (RT) activity and p24 production. On day 11 post‐infection, CRF02_AG strains had a 1.4–1.9 times higher RT activity and reached a significantly higher level of p24 production than the parental subtypes A and G. Furthermore, the replication rate as measured by p24 production was 1.4 times higher for CRF02_AG strains compared to the subtypes A and G. This study suggests that the recombination event that led to CRF02_AG resulted in a variant with a better replicative capacity than its progenitors. This adaptation could contribute to the broader spread of HIV‐1 CRF02_AG leading to its predominance in West Central Africa compared to the lower prevalence of its parental subtypes A and G. J. Med. Virol. 78:523–534, 2006. © 2006 Wiley‐Liss, Inc.

Url:

https://api.istex.fr/document/A113A23D17C4AC3A743C615B17AE8F2A0E7E62B5/fulltext/pdf

DOI: 10.1002/jmv.20572

Affiliations:

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Le document en format XML

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<term>Grant sponsor</term>
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<term>Burda</term>
<term>Cameroon</term>
<term>Cameroonian</term>
<term>Carr</term>
<term>Coreceptor usage</term>
<term>Culture medium</term>
<term>Cytotoxicity</term>
<term>Disease progression</term>
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<term>Grant number</term>
<term>Grant sponsor</term>
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<term>Heyndrickx</term>
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<term>Human virus type</term>
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<term>Konings</term>
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<term>Phylogenetic analysis</term>
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<front><div type="abstract" xml:lang="en">Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form (CRF) 02_AG is the predominant subtype in Cameroon, even more prevalent than the parental subtypes A and G. An important question that needs to be addressed is whether recombination in HIV‐1 infection can lead to the emergence of viruses with biological advantages. The replicative capacity was investigated in peripheral blood mononuclear cells (PBMCs) of 13 R5‐tropic primary HIV‐1 isolates, including 5 CRF02_AG, 4 subtype A, and 4 subtype G viruses. HIV‐1 subtype identity was defined by phylogeny either of the full‐length genome or analysis of a combination of segments of the gag, pro, pol, and env genes followed by recombination breakpoint analysis. All viruses were grown on PBMCs for 11 days and culture supernatant was analyzed for reverse transcriptase (RT) activity and p24 production. On day 11 post‐infection, CRF02_AG strains had a 1.4–1.9 times higher RT activity and reached a significantly higher level of p24 production than the parental subtypes A and G. Furthermore, the replication rate as measured by p24 production was 1.4 times higher for CRF02_AG strains compared to the subtypes A and G. This study suggests that the recombination event that led to CRF02_AG resulted in a variant with a better replicative capacity than its progenitors. This adaptation could contribute to the broader spread of HIV‐1 CRF02_AG leading to its predominance in West Central Africa compared to the lower prevalence of its parental subtypes A and G. J. Med. Virol. 78:523–534, 2006. © 2006 Wiley‐Liss, Inc.</div>
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	Le SIDA au Ghana (serveur d'exploration)
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Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form 02_AG (CRF02_AG) has a higher in vitro replicative capacity than its parental subtypes A and G

Human immunodeficiency virus type 1 (HIV‐1) circulating recombinant form 02_AG (CRF02_AG) has a higher in vitro replicative capacity than its parental subtypes A and G

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri